| Table 1. Effect of different doses of HEE of C. pulcherrima on the activities of SOD and CAT in the testis, epididymis, sperm pellet and liver after 2 hrs of incubation. Data expressed as mean±SEM, n=16. Values in columns with different superscripts (a-c) differ from each other significantly; p<0.05. ANOVA followed by “Multiplecomparison Student’s two-tail ‘t’-test. Values in parenthesis indicate the percentage of diminution (↓) in respect to the control. | ||||||||||
| Experimental groups | Anti-oxidative enzymes | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| SOD (unit/mg of tissue) | CAT (μM of H2O2 consumed / mg of tissue/min) | |||||||||
| Reproductive tissue | Sperm pellet (Rat) |
Sperm pellet (Human) |
Metabolic tissue (liver) (Rat) |
Reproductive tissue | Sperm pellet (Rat) |
Sperm pellet (Human) |
Metabolic tissue (liver) (Rat) |
|||
| Testis (Rat) |
Epididymis (Rat) |
Testis (Rat) |
Epididymis (Rat) |
|||||||
| Control | 1.44±0.07a | 1.55±0.06a | 2.51±0.19a | 9.67±0.31a | 2.16±0.05a | 2.56±0.12a | 1.86±0.09a | 4.58±0.05a | 38.57±1.80a | 8.23±0.71a |
| 1 mg HEE- exposure group |
1.32±0.02a (8.33%↓) |
1.42±0.02a (8.38%↓) |
1.42±0.09b (43.42%↓) |
8.59±0.54a (11.16%↓) |
1.98±0.06a (8.33%↓) |
2.41±0.07a (5.85%↓) |
1.82±0.09a (2.15%↓) |
3.16±0.05b (31%↓) |
37.44±1.31a (2.92%↓) |
7.63±0.51a (7.29%↓) |
| 2 mg HEE- exposure group |
0.88±0.03b (38.88%↓) |
0.93±0.04b (40%↓) |
0.92±0.07b (63.34%↓) |
6.55±0.37b (32.26%↓) |
1.85±0.18a (14.35%↓) |
1.98±0.06b (22.65%↓) |
1.4±0.09b (24.73%↓) |
2.34±0.03c (48.90%↓) |
27±1.11b (29.99%↓) |
7.12±0.78a (13.48%↓) |
| 4 mg HEE- exposure group |
0.82±0.02b (43.05%↓) |
0.88±0.03b (43.22%↓) |
0.85±0.07b (66.13%↓) |
5.01±0.21c (48.19%↓) |
1.88±0.11a (12.96%↓) |
1.88±0.06b (26.56%↓) |
1.33±0.08b (30.10%↓) |
2.25±0.09c (50.87%↓) |
21±0.97c (45.55%↓) |
6.98±0.96a (15.18%↓) |