| Table 1. Fertilization outcomes and embryo development outcomes on sibling oocytes, comparing ZyMōt™ to density gradient centrifugation (DGC). Clinically useful embryos refer to high-quality blastocysts, ≥3BB, transferred fresh or vitrified for later. KID-score and iDA score are expressed as median and range. The time lapse parameters are expressed as mean and standard deviation, hours post insemination (HPI). | |||
| Parameter | ZyMōt™ | DGC | p-value |
|---|---|---|---|
| Retrieved ocytes (n) | 69 | 74 | - |
| M2 oocytes (n) | 62 | 67 | - |
| Maturity rate (%) | 89.9 | 90.5 | .89 |
| 2PN (n) | 48 | 52 | - |
| 2PN rate (%) | 77.4 | 77.6 | .98 |
| Semen parameters (after processing) Progressive motile sperms (%) Progressive motile sperms (millions) |
97.2±0.8 31.7 |
88.4±7.4 17.6 |
.002 .102 |
| Embryo development parameters Clinically useful embryos (n) Utility rate (%) KID-Score D5 (day 5 only) iDAScore (day 5 only) |
23 47.9 7.1 (2.9-9-7) 8.7 (3.3-9.7) |
27 51.9 7.3 (5.3-9.4) 8.2 (4.4-9.7) |
- .69 .77 .82 |
| Time lapse parameters* tPNf t2 t3 t4 t5 t6 t7 t8 t9+ tSC tM tSB tB tEB tHB |
24.5±3.8 27.1±3.5 38.5±5.8 41.8±7.6 52.5±7.6 54.3±8.2 57.0±9.8 60.4±11.5 73.7±8.2 80.1±6.5 88.0±7.2 98.2±7.6 108.7±12.7 112.3±7.7 114.8±9.9 |
23.6±3.8 27.1±4.6 37.0±7.5 41.7±12.0 51.4±9.5 54.2±7.2 57.9±9.3 60.4±9.0 73.2±7.0 81.5±7.6 86.3±7.8 95.9±6.4 105.7±8.5 113.2±7.2 118.6±9.9 |
0.296 0.966 0.312 0.944 0.604 0.947 0.699 0.987 0.813 0.537 0.400 0.225 0.324 0.710 0.362 |
PN=pronuclei, tPNf=time of PN fading, tSC=time of starting compaction, tM=time to morula, tSB=time of starting blastulation, tB=time to blastocyst, tEB=time to expanded blastocyst, tHB=time to hatching blastocyst. *= number of observations vary between parameters, missing data for later stages due to arresting embryos.