| Table 2. Developmental competence of the oocytes exposed to prednisolone (PRDL) in vivo during superovulation protocol (number of oocytes/embryos in parentheses). | |||||||||
| Group | Hours post insemination (hpi) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 10 | 24 | 48 | 72 | 96 | |||||
| 2PN/2PB | 2 Cell | 4 Cell & below | 6-8 cell | Cleavage stage | Compacted | Early Blastocyst |
Expanded | Hatching | |
| Control (n=142) |
91.07±4.98 (n=128) |
96.87±3.13 (n=121) |
50.26±12.52 (n=59) |
49.73±12.52 (n=69) |
5.9±2.9 (n=09) |
94.1±2.96 (n=119) |
16.9±2.13 (n=20) |
22.97±10.23 (n=25) |
46.87±10.99 (n=70) |
| Vehicle control(VC) (n=135) |
75.7±13.68 (n=113) |
78.87±9.12 (n=98) |
52.46±6.89 (n=64) |
47.53±6.89 (n=49) |
23.76±8.5 (n=21) |
76.23±8.50 (n=92) |
5.96±3.23 (n=05) |
15.6±3.37 (n=15) |
51.27±9.09 (n=66) |
| 0.01mg/kg PRDL (n=183) |
61.27±11.02 (n=120) |
93.73±5.25 (n=119) |
49.03±0.52 (n=57) |
51.61±1.13 (n=63) |
4.93±2.94 (n=05) |
96.06±3.4 (n=115) |
39.33±8.24 (n=30) |
22.3±7.04 (n=20) |
36.06±16.65 (n=57) |
| 0.1mg/kg PRDL (n=157) |
74.93±7.75 (n=118) |
90.17±6.38 (n=106) |
34.4±14.38 (n=39) |
65.6±14.38 (n=79) |
14±2.81 (n=17) |
86±2.80 (n=101) |
15.83±3.56 (n=18) |
28.8±5.46 (n=32) |
40.7±5.55 (n=50) |
| 1mg/kg PRDL (n=123) |
83.76±8.19 (n=102) |
97.33±1.41 (n=100) |
28.86±11.23 (n=31) |
71.13±11.23 (n=71) |
3.76±1.94 (n=03) |
96.23±1.94 (n=99) |
8.5±3.21 (n=08) |
17.03±6.4 (n=16) |
70.23±10.99 (n=73) |
Data are expressed as numbers and Mean ± Standard Error of Mean from minimum of three trials conducted.