Figure 2. Relative expression fold change of CLDN4 at three different treatment times (A: 24h, B: 48h, and C: 72h). Gene expression results are normalized to β-actin mRNA amplification. A: **vs. control and 200nM (p=0.000); # vs. 50nM (p=0.01); * vs. control (p=0.01); Ø vs. 200nM (p=0.000); α vs. 200nM (p=0.03); B: ** vs control, 50nM, and 200nM (p=0.000); * vs. control (p=0.004); Ø vs. 200nM (p=0.000); C: ** vs. control (p=0.003); # vs. control (p=0.02); * vs. control (p=0.04); D: CLDN4 expression process within each group through study course; control (* vs. 72h (p=0.04); 100nM (# vs. 24h (p=0.001), ** vs. 72h (p=0.000)); 200nM (Ø vs. 24h and 48h (p=0.005)).