Figure 4. Relationship between patient age and key reproductive parameters assessed. (A) Antral follicle count (AFC) significantly decreases with advancing age. (B) A negative correlation is observed between the number of oocytes retrieved during ovarian puncture and age. (C) A negative correlation is also evident between the number of mature oocytes and age. (D) The number of blastocysts formed shows a significant decline with increasing age. r=Spearman’s correlation coefficient; No.=number. Significant differences are noted when – p-value<0.05.