| Table 2. Sperm motility, viability, morphology, ROS levels, and DNA integrity in controls and post-cryopreserved samples in different media and methods. | |||||
| Parameters (n=40) |
Control | Vitrification | Liquid nitrogen vapor | ||
|---|---|---|---|---|---|
| In-house-V | Simp-V | Com-L | Simp-L | ||
| Total motility (%) | 92.4 (85.4-95.3)* |
77.8 (70.8-81.3) |
58.8 (51.4-63.3)† |
34.5 (27.5-43.6) |
57.6 (53.2-68.9)‡ |
| Progressive motility (%) | 89.2 (84.5-93.0)* |
67.5 (60.0-72.0) |
49.0 (41.1-55.3)† |
28.5 (21.7-34.9) |
50.6 (42.9-56.3)‡ |
| Viability (%) | 90.5 (86.0-93.0)* |
75.5 (68.0-83.0) |
59.0 (53.3-66.0)† |
37.0 (29.3-46.0) |
61.0 (52.0-67.8)‡ |
| Normal morphology (%) | 14.9 (8.3-20.5) |
12.3 (5.3-16.2) |
12.0 (9.0-16.9) |
11.1 (7.4-16.3) |
12.4 (7.6-16.6) |
| ROS (RLU/sec/106) | 4.5 (2.3-8.2)§ |
7.4 (2.3-16.5) |
8.7 (3.4-15.6) |
11.7 (5.9-18.7) |
8.1 (4.2-13.4) |
| DNA fragmentation (%) | 24.5 (14.3-30.0) |
25.5 (19.3-32.0) |
27.5 (21.3-37.8) |
30.0 (20.0-36.0) |
26.5 (23.0-29.0) |
Data expressed as median (interquartile range)
Friedman’s test, *p<0.001 vs. all groups (In-house-V, Simp-V, Com-L, Simp-L), †p<0.001 vs. In-house-V, ‡p<0.001 vs. Com-L, §p<0.05 vs. all groups (In-house-V, Simp-V, Com-L, Simp-L)
Abbreviation: In-house = in-house media, Simp = simplified sucrose media, Com = commercial media, V = vitrification, L = liquid nitrogen vapor freezing, ROS = reactive oxygen species.