| Table 3. Sperm kinemetics in controls and post-cryopreserved samples in different media and methods. | |||||
| Parameters (n=40) |
Control | Vitrification | Liquid nitrogen vapor | ||
|---|---|---|---|---|---|
| In-house-V | Simp-V | Com-L | Simp-L | ||
| VAP (µm/sec) | 72.9 (67.0-79.5)* |
60.4 (55.3-68.3) |
57.2 (51.8-60.1) |
54.5 (49.5-59.0) |
56.7 (53.1-62.5) |
| VSL (µm/sec) | 61.5 (53.3-66.8)* |
52.5 (46.8-57.3) |
48.8 (42.7-51.3) |
44.8 (41.1-49.9) |
47.8 (43.9-51.9) |
| VCL (µm/sec) | 116.2 (110.3-130.1)* |
100.6 (92.8-116.7) |
97.5 (85.4-109.0) |
93.9 (86.5-101.9) |
96.2 (88.3-106.0) |
| ALH (µm) | 5.9 (5.5-6.5)* |
5.0 (4.6-6.1) |
5.0 (4.5-5.6) |
5.0 (4.4-5.4) |
5.0 (4.6-5.6) |
| BCF (Hz) | 25.7 (23.2-27.9)† |
27.5 (25.8-30.2) |
28.0 (26.3-30.9) |
32.3 (29.3-36.0) |
28.4 (26.9-30.0)‡ |
| STR (%) | 82.1 (77.7-85.4) |
82.8 (80.4-86.3) |
81.1 (79.8-84.6) |
81.5 (77.8-84.9) |
82.6 (78.8-84.1) |
| LIN (%) | 53.2 (47.5-58.4)† |
50.2 (46.9-54.2) |
48.3 (45.8-52.4) |
48.2 (45.3-52.8) |
49.2 (45.8-51.9) |
Data expressed as median (interquartile range)
Friedman’s test, *p<0.001 vs. all groups (In-house-V, Simp-V, Com-L, Simp-L), †p<0.001 vs. Simp-V, Com-L and Simp-L, ‡p=0.003 vs. Com-L
Abbreviation: In-house = in-house media, Simp = simplified sucrose media, Com = commercial media, V = vitrification, L = liquid nitrogen vapor freezing, VAP = velocity of smooth average path, VSL = mean straight-line velocity, VCL = mean curvilinear velocity, ALH = amplitude of lateral head displacement, BCF = beat cross frequency, STR = percent straightness, LIN = percent linearity