Table 1. Primer of interested gene.
Gene	Forward Primer	Reverse Primer	Annealing temp. (°C)	Amplicon size (bp)
FUT4	5'- AGGGGGTTCTTCCTCACCTT -3'	5'- ATATGGCCTGTGGCAGATGG -3'	59	105
CDH2	5'- GACGGTTCGCCATCCAGAC -3'	5'- TCGATTGGTTTGACCACGG -3'	56	67
SOX9	5'- AGCGAACGCACATCAAGAC -3'	5'- GCIGTAGTGTGGGAGGTTGAA -3'	58	110
LGR5	5'- CACCTCCTACCTAGACCTCAGT -3'	5'- CGCAAGACGTAACTCCTCCAG -3'	61	94
POU5F1	5'- ACATCAAAGCTCTGCAGAAAGAACT -3'	5'-CTGAATACCTTCCCAAATAGAACCC -3'	56	127
PDGFRB	5'- TGGTGCTCACCATCATCTCC -3'	5'- CACCTTCCATCGGATCTCGTAA -3'	59	80
SUSD2	5'- CAGCTACCAGAGATGGCGAGTGG -3'	5'- CCTCTCGGAAGTCATCGCTCAGG -3'	61	134
MCAM	5'- ATCGCTGCTGAGTGAACCACAG -3'	5'- CTACTCTCTGCCTCACAGGTCA -3'	58	123
ACTB	5'- ATGTGGCCGAGGACTTTGATT -3'	5'- AGTGGGGTGGCTTTTAGGATG -3'	58	107