Table 1. Included studies
Study Country/Center Enrolment Period Embryos Intervention Control
Balaban et al. , 2004 Turkey, American Hospital of Istanbul 2006.Jun to 2007.Feb Fresh Blastocyst N=193, Embryoglue
(30 min)		 N=193, G2 + HSA
Chen et al. , 2001 Taiwan, IVF-Unit, Dr. Tsai & Dr. Chen’s Women Hospital Unclear Unclear N=35,
HSA + HA		 N=35,
HSA
Child et al. , 2021 United Kingdom, TFP Oxford Fertility, Oxford 2017 to 2019 Fresh or frozen
transfer of 1-2 cleavage stage embryos or
blastocysts.		 N=343 Embryoglue
(10-30min)		 N=338 COOK
culture media
Dittmann-Müller et al. , 2009 Chemnitz, Germany,
Women’s Hospital, IVF,
Basel, Switzerland, Fertilitas, IVF/ICSI Centre,
Würzburg, Germany,
University Hospital Würzburg, Dept. of Obstetrics and Gynecolgy		 2006.Jan to 2007.Mar Fresh D3 embryos N=54,
EmbryoGlue (30min)		 N=48,
G-1 and G-2 version
3 plus + 10% HSA
Drew et al. , 2014 Unclear Unclear D3 or D5 embryos,
single or double		 N=Unclear
EmbryoGlue (10min)		 N=Unclear
Standard media
Fancsovits et al. , 2015 Budapest, Hungary, Division
of Assisted Reproduction,
Semmelweis University		 2010.Jan to 2012.Aug D2 ou D3,
fresh embryos		 N=290, EmbryoGlue (10min) N=291,
G-2+ (Vitrolife)
Fasano et al. , 2016 Brussels, Belgium Hôpital
Erasme, ULB, FIV Laboratoire		 2015.Jun to 2016.Jan Day2/Day3 and
Day5/Day6		 N=136
Embryo-Glue		 N=192
Routine ET medium
Friedler et al. , 2005 Tel Aviv, Israel, IVF and Infertility Unit, Assaf Harofeh Medical Center Tel Aviv University Unclear D2 Embryos N=94,
Embryo-Glue		 N=93,
HTF medium + 20% SSS
Friedler et al. , 2007 Israel, Assaf Harofeh Medical Center 2005.Jun to 2006.Nov D2 ou D3,
fresh embryos		 N=51,
EmbryoGlue		 N=50,
HTF medium with gentamicin + 20% SSS
Hazlett et al. , 2008 Elgin, IL, Sherman
Hospital		 Unclear D3 or D5
fresh embryos		 D3=85
D5=32
EmbryoGlue (10-60min)		 D3=79
D5=29
D3=IVC-1 or IVC-2
+ 0.5% HSA
D5= G2.3 + 0.5% HSA
Kandari, 2019 Mumbai, India, Cellsure Biotech and Research Centre 2017.Jan to 2018.Aug Cleavage stage or blastocyst single fresh N=153, EmbryoGlue N=168,
CSCM medium
Khan et al. , 2004 Michigan, USA, IVF
Michigan, Rochester Hills, IVF and Andrology
Laboratory		 Unclear D3 fresh embryos N=Unclear
EmbryoGlue (10min)		 N=Unclear
P1 Complete medium
Korosec et al. , 2007 Ljubljana, Slovenia,
Department of Obstetrics and Gynecology,
University Medical Centre Ljubljana.
Bregenz, Austria, Institute for Reproductive Medicine and Endocrinology, Roemerstrasse		 2004.Apr to 2006.Jun Fresh and frozen
single blastocyst transfer		 N=28 (fresh)
N=102 (frozen)
EmbryoGlue		 N=37 (fresh)
N=112 (frozen)
M2 medium
Mahani & Davar, 2007 Yazd, Islamic Republic of Iran, Research and Clinical Centre for Infertilityin 2003.Set to 2004.Jan D3 Fresh embryos N=30
EmbryoGlue (10min)		 N=30
Medium containing
albumin 20%
Morbeck, 2007 Mayo Clinic, Rochester,
Minnesota, USA		 2003.May to 2005.Jun Frozen embryo
transfers		 N=41
Embryoglue		 N=42
Standard medium
Ravhon et al. , 2005 Israel, The Edith Wolfson
Medical center		 2004.Jul to 2004.Nov Fresh embryo
transfers		 N=79
EmbryoGlue (10min)		 N=69
Medium G-1
Schoolcraft et al. , 2002 Englewood, Colorado; Colorado Ctr for Reproductive Medicine 2001.Jan to 2002.Feb D3 fresh embryos N=91
EmbryoGlue		 N=84
Medium G2
Ten et al. , 2019 Alicante, Spain, Department of Reproductive Medicine, Instituto Bernabeu 2018.Mar to 2019.Jan D5 Fresh single
embryo transfer		 N=48 (first
randomization)
EmbryoGlue		 N=61 (first
randomization)
Global Total (GT)®,
LifeGlobal®
Sellers et al. , 2022 Alicante, Spain, Department of Reproductive Medicine, Instituto Bernabeu 2018.Mar
to 2019.Jan		 D5 Fresh single
embryo transfer		 N=104 (second
randomization)
EmbryoGlue		 N=89 (second
randomization) Continuous Single Culture® Complete
(CSCC)
Simon et al. , 2003 Jerusalem, Israel, IVF unit, Departament of Obstretics and Gynecology, Hadassah
University Hospital Ein Kerem		 Unclear D3 Fresh embryos N=40,
P1 medium supplemented with 0.5 mg/ml of highly purified fermentation-based hyaluronic acid (5-10min)		 N=40,
P1 Medium
containing 10%of SSS
Urman et al. , 2008 Istanbul, Turkey, Assisted
Reproduction Unit, American Hospital of Istanbul		 2006.Jun and 2007.Feb D3 or D5
fresh embryos		 D3=412
D5=227
EmbryoGlue (30min)		 D3=413
D5=230
G2, version 3 +
HSA
Walker et al., 2005 Rochester, MN, Mayo Clinic College of Medicine,
London, United Kingdom, London Fertility Centre		 Unclear Frozen clevage
embryo transfer		 N=34
EmbryoGlue®		 N=34
G1.3 media
Yakin et al., 2001 Istanbul, Turkey, VKV
American Hospital, Assisted Reproduction Unit		 Unclear Frozen-thawed
embryo		 N=65,
EmbryoGlue		 N=64,
G2 culture medium
Yung et al. , 2021 Hong Kong
The Centre of Assisted Reproduction and
Embryology,
The University of Hong Kong -Queen Mary Hospital,
The Dr. Stephen Chow Chun-kay Assisted
Reproduction Centre, Kwong
Wah Hospital		 2016. Apr to 2018.Apr Frozen Cleavage
stage embryos or
blastocysts		 N=275
EmbryoGlue		 N=275
G-2 medium
(Vitrolife)

HSA=Human serum albumin solution; HA=Hyaluronic Acid; IVC=In VitroCare; SSS= Synthetic serum substitute; HTF=human tubal fluid