Figure 2. Effect of high-fat diet-induced obesity on (A) lipid droplet accumulation in oocytes from control (n=27) and obese group (n=29), assessed by Nile red staining; (B) representative images showing low and high lipid accumulation in GV oocytes (400x magnification); (C) survival of GV oocytes after vitrification and thawing (control: n=130; obese: n=101); (D) nuclear maturation potential in GV oocytes after vitrification and thawing (fresh control: n=79, obese: n=50; vitrified control: n=35, obese: n=42); (E) intracellular reactive oxygen species (ROS) levels in GV oocytes (fresh control: n=11, obese: n=8; vitrified control: n=11, obese: n=11); (F) representative images showing intracellular ROS levels in GV oocytes stained with 2,7-dichlorodihydrofluorescein diacetate (400x magnification). The scale bar equals 20 µm. Data are presented as mean ± SEM. Control: Obese: