Figure 3. Effect of high-fat diet on organelle distribution patterns in GV oocytes from fresh and vitrified-thawed samples. Percentage of GV oocytes (n=23 oocytes/group) showing an aggregated distribution pattern of (A) ER; (B) and representative images of different ER distribution patterns in oocytes stained with ER-tracker (400x magnification); (C) mitochondrial distribution pattern; (D) representative images of different mitochondrial distribution patterns in oocytes stained with Mito-tracker (400x magnification). The scale bar indicates 20 µm. Control: Obese: