Figure 4. Effect of high-fat diet on meiotic spindle organization in MII oocytes derived from IVM of fresh and vitrified-thawed GV oocytes. (A) Percentage of MII oocytes with spindle damage (fresh-control: n=26, obese: n=25; VT-control: n=22, obese: n=18); (B) Representative images from (a, b) oocytes with normal spindle organization, and (c-m) oocytes with abnormal spindle organization (400x magnification). The scale bar indicates 20 µm. Control: Obese: