Figure 5. Effect of a high-fat diet and vitrification on the expression of ER stress markers in GV oocytes. (A) Expression of GRP78 in oocytes (fresh-control: n=30 and obese: n=30, VT-control: n=30 and obese: n=29); (B) Expression of XBP1 in oocytes (fresh-control: n=30 and obese: n=33, VT-control: n=30 and obese: n=30); (C) Expression of ATF6 in oocytes (fresh-control: n=30 and obese: n=32, VT-control: n=30 and obese: n=32); (D) Expression of ATF4 in oocytes (fresh-control: n=30 and obese: n=28, VT-control: n=30 and obese: n=33); (E) Representative images showing GRP78, XBP1, ATF6, and ATF4 expression in GV oocytes; (400x magnification). The scale bar represents 20 µm. The data are presented as mean ± SEM. Control: Obese: