Figure 1. Real-time PCR analysis of ZNF83 (zinc Finger 83), ACY-1 (aminoacylase-1), and SMC5 (structural maintenance of chromosomes 5) genes expression in non-fertilized groups versus fertilized group. Expression level of all three genes (ACY-1, SMC5, ZNF83) were significantly downregulated in the non-fertilized group compared to the fertilized group (p<0.0001****). β-actin was used for normalization. Number of biological replicates (n): 3.