Figure 2. Western blot analysis ofZNF83 (zinc Finger 83), ACY-1 (aminoacylase-1), and SMC5 (structural maintenance of chromosomes 5) expression. Protein levels of three target (ACY-1, SMC5, ZNF83) showed significant downregulation in the non-fertilized group compared to the fertilized group (*p<0.05/ **p<0.01). Molecular Weight: ZNF83: 60kda, ACY-1:46kda, SMC5:129kda, β-actin:42kda. β-actin was used for normalization. Number of biological replicates (n):2.