Figure 2. Western blot analysis of LC3B and p62 expression in ovarian granulosa cells from ND, HFD, and SE groups(A) Representative Western blot bands of LC3B-I and LC3B-II in GCs (ND, n=6; HFD, n=6; SE, n=6).(B) Representative Western blot bands of p62 in GCs (ND, n=5; HFD, n=6; SE, n=6).(C) Grayscale quantification of LC3B-II/I ratio.(D) Grayscale quantification of p62 expression level.Data are expressed as mean ± SEM. Significant differences are indicated as follows: *p<0.05, **p<0.01