Figure 4. Western blot analysis of p-mTOR/mTOR and p-RPS6/RPS6 expression in ovarian granulosa cells under obesity and exercise-induced weight loss conditions(A) Representative Western blot bands of p-mTOR/mTOR in GCs (ND, n=7; HFD, n=7; SE, n=7).(B) Representative Western blot bands of p-RPS6/RPS6 in GCs (ND, n=7; HFD, n=7; SE, n=7).(C) Grayscale quantification of p-mTOR/mTOR expression ratio.(D) Grayscale quantification of p-RPS6/RPS6 expression ratio.Data are expressed as mean ± SEM. Statistical significance is indicated as follows: *p<0.05, **p<0.01.