JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):20
Oral Presentation
29th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263541
P-08. An exception to the "haste makes waste" proverb: euploid embryos are faster to form complete blastocoel and full blastocyst
Bruna Rabello Iglesias1, Rebekka Rae Him Kim1, Ana Beatriz Nogueira Pacheco2, Natália Paes Barbosa Valadares2, Iris Oliveira Rocha2, Luiz Carlos Pinheiro Junior2, Adelino Amaral Silva2, Bruno Ramalho Carvalho1
1 Faculdade de Ciências da Educação e Saúde do Centro Universitário de Brasília (FACES/CEUB) - BRASÍLIA - DF - Brasil
2 Genesis Centro de Assistência em Reprodução Humana - Brasília - DF - Brasil
Objective: To identify whether there is a correlation between morphokinetic parameters and ploidy of human blastocysts, seeking to develop a non-invasive evaluation strategy that can predict the existence of aneuploidy.
Methods: Observational, prospective study, assessing time-lapse images of 43 developing human blastocysts further allocated to preimplantation genetic testing for aneuploidy (PGT-A). Embryo scoring using the automated decision support tool KIDScore was also evaluated. Normally distributed results were analyzed using the Welch test, and nonparametrically distributed results were analyzed using the Mann-Whitney test. Areas under the receiver operating characteristic curves (AUC-ROC) were obtained to assess morphokinetic parameters capacity to predict aneuploidy. The significance level was set at p<0.05 in all analyses.
Results: The time to full blastocyst formation (tB) and the interval between initial blastocoel identification and complete blastocyst formation (tB - tSB) were shorter for euploid blastocysts. The respective mean/median times/intervals (in hours) for euploid and aneuploid embryos were as follows: tB, 101.5 vs. 109.5 (p=0.0011); tB-tSB, 6.1 vs. 11.5 (p=0.0327). The KIDScore was also significantly higher among euploid embryos when compared to aneuploid ones (means, 7.5 vs. 5.1, respectively, p=0.0002). A moderate predictive capacity for identifying aneuploid embryos was demonstrated for the tB parameter and the KIDScore score: tB, AUC-ROC=0.7357 (95% CI 0.5887 – 0.8828, p=0.0116); KIDScore AUC-ROC=0.8370 (95% CI 0.7007 – 0.9734, p=0.0003).
Conclusion: The time to complete blastocoel and full blastocyst formation was shorter for euploid than for aneuploid embryos, suggesting that late embryonic development may be a signature of chromosomal abnormalities. If confirmed by studies with a larger sample size, our results could be used to obtain informed decisions about whether to perform embryo biopsies, avoiding them in blastocysts with a high probability of euploidy.