JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):20
29th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263541
JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):20
Oral Presentation
29th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263541
P-08. An exception to the "haste makes waste" proverb: euploid embryos are faster to form complete blastocoel and full blastocyst
1 Faculdade de Ciências da Educação e Saúde do Centro Universitário de Brasília (FACES/CEUB) - BRASÍLIA - DF - Brasil
2 Genesis Centro de Assistência em Reprodução Humana - Brasília - DF - Brasil
Objective: To identify whether there is a correlation between morphokinetic parameters and ploidy of human blastocysts, seeking to develop a non-invasive evaluation strategy that can predict the existence of aneuploidy.
Methods: Observational, prospective study, assessing time-lapse images of 43 developing human blastocysts further allocated to preimplantation genetic testing for aneuploidy (PGT-A). Embryo scoring using the automated decision support tool KIDScore was also evaluated. Normally distributed results were analyzed using the Welch test, and nonparametrically distributed results were analyzed using the Mann-Whitney test. Areas under the receiver operating characteristic curves (AUC-ROC) were obtained to assess morphokinetic parameters capacity to predict aneuploidy. The significance level was set at p<0.05 in all analyses.
Results: The time to full blastocyst formation (tB) and the interval between initial blastocoel identification and complete blastocyst formation (tB - tSB) were shorter for euploid blastocysts. The respective mean/median times/intervals (in hours) for euploid and aneuploid embryos were as follows: tB, 101.5 vs. 109.5 (p=0.0011); tB-tSB, 6.1 vs. 11.5 (p=0.0327). The KIDScore was also significantly higher among euploid embryos when compared to aneuploid ones (means, 7.5 vs. 5.1, respectively, p=0.0002). A moderate predictive capacity for identifying aneuploid embryos was demonstrated for the tB parameter and the KIDScore score: tB, AUC-ROC=0.7357 (95% CI 0.5887 – 0.8828, p=0.0116); KIDScore AUC-ROC=0.8370 (95% CI 0.7007 – 0.9734, p=0.0003).
Conclusion: The time to complete blastocoel and full blastocyst formation was shorter for euploid than for aneuploid embryos, suggesting that late embryonic development may be a signature of chromosomal abnormalities. If confirmed by studies with a larger sample size, our results could be used to obtain informed decisions about whether to perform embryo biopsies, avoiding them in blastocysts with a high probability of euploidy.