JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):63
Poster Presentation
29^th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263604

P-51. Correlation Between Sperm DNA Fragmentation and Motility Parameters: A Comprehensive Study

Marcello Machado Gava¹, Yasser Omar Dalle¹, Andre Marantes Masciarelli Pinto¹, Milton Ghirelli Filho¹, Pedro Humberto Filho¹, Emerson Barchi Cordts², Bruna Bizio Parra², Ivan Henrique Yoshida², Isabela Oliveira e Silva Barion¹, Caio Parente Barbosa², Sidney Glina¹

¹ Disciplina de Urologia do Centro Universitário FMABC - Santo Andre - SP - Brasil
² Instituto Ideia Fertil - Santo Andre - SP - Brasil

Objective: To investigate the correlation between sperm DNA fragmentation (SDF) and motility parameters, aiming to enhance the understanding of male infertility factors and improve diagnostic approaches.
Methods:
Design: Retrospective observational study.
Setting: Single andrology center in Brazil.
Patients: A total of 590 semen samples from men aged 18–50 years undergoing routine fertility assessments between August 2021 and February 2024.
Main Outcome Measures: Sperm DNA fragmentation assessed by the Sperm Chromatin Dispersion (SCD) test and motility parameters (rapid progressive motility [MPR], slow progressive motility [MPL], non-progressive motility [MNP], immotility [IMOV]) evaluated according to WHO guidelines. Total motile sperm count (TMSC) was calculated. Statistical correlations between SDF, motility parameters, and TMSC were analyzed.
Results: The mean SDF value was 23.5%. Pearson’s correlation revealed a moderate negative correlation between SDF and rapid progressive motility (r = -0.XX, p<0.05), and a moderate positive correlation with the percentage of immotile sperm. Samples were categorized into three groups based on SDF (<20%, 20–30%, >30%). Significant differences in MPR, immotility, and sperm morphology were found between groups with normal and altered SDF (p<0.01). Higher DNA fragmentation was associated with lower TMSC and reduced sperm motility, while immotility increased with SDF. These findings support an inverse relationship between sperm DNA integrity and motility parameters.
Conclusion: This study demonstrates a significant inverse correlation between sperm DNA fragmentation and motility, highlighting SDF as a potential key parameter in male fertility assessment. Incorporating DNA fragmentation analysis alongside conventional semen parameters may improve infertility diagnostics. Further prospective studies are warranted to assess the impact of these correlations on reproductive outcomes.