JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):70
Oral Presentation
29^th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263632

P-58. Diagnosis of male infertility: analysis of spermogram and Sperm Chromatin Structure Assay (SCSA) results

Raissa Ramos Coelho Penha¹, Gabriela Cristina Fonseca Almeida¹, Édlla Mikaine Padre e Fechine¹, Mellissa Nauhanne Roxo Leite¹, Fulvia Estefânia Padre e Fechine¹, Patrícia Tourinho da Silva¹

¹ FertVida São Luís - São Luís - MA - Brasil

Objective: The present study aimed to evaluate the diagnoses of male infertility of patients who attended an Assisted Human Reproduction clinic.
Methods: The data used in this study is from the spermogram (seminal analises) and from the Sperm Chromatin Structure Assay (SCSA) reports of patients who underwent the tests at a private Human Reproduction clinic in Brazil, from January 2024 to July 2025. The sum (total), average (mean), standard deviation (sd) and percentage were performed in Microsoft Excel. The Student's t-test was used to evaluate if there was a statistically significant relationship between age and diagnosis (normal or abnormal). A statistical significance level of α=0.05 and a confidence interval of 95% were applied to the tests. Statistics were performed using the GraphPad program.
Results: As a result, 164 spermogram tests and 30 SCSA were evaluated. The age mean of patients who underwent the tests was 38.7 (sd, ±7) years old and 78% of these individuals were in the age group of 35 to 67 years old. 37.8% of patients presented normal semen analysis, while 62.2% had some abnormality in the report. No significant difference was observed between age and diagnosis (t=1.9142, df=163, p=0.0573). In the results of morphological analysis, the following diagnoses were found: azoospermia (5.5%), teratozoospermia (25.6%), oligoasthenoteratozoospermia (8.5%), oligoteratozoospermia (7.9%), asthenoteratozoospermia (3%), oligozoospermia (7.9%) and asthenozoospermia (3%). In addition to the morphological alterations, 6.5% of patients also presented necrozoospermia. Regarding the SCSA results, 33% were positive, 3 of patients who had a normal spermogram result, and 7 patients with altered semen in both exams.
Conclusion: In a conclusion, the data revealed teratozoospermia as the most frequent sperm alteration found. No relationship was observed between age and patient diagnosis. Even when the spermogram test is within parameters considered normal, the SCSA test showed abnormalities. Therefore, the indication of SCSA as an extended analysis of semen can help in the diagnosis of infertility in patients.