JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):80
Poster Presentation
29th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263659

P-68. Effect of Hyaluronic Acid-Enriched Media (EmbryoGlue®) in Embryo Implantation and Clinical Pregnancy rate in good prognosis patients

Lorena Ana Mercedes Lara Urbanetz1, Luana Carolina Oliveira Rodrigues2, Luiza Mansini Quadros Bezerra3, Giulia Nunes Victorello3, Priscila Luiza dos Santos3, Luciana Issas de Oliveira2, Joji Ueno1

1 Clinica Gera - São Paulo - SP – Brasil
2 Clinica Gera/LabFIV - São Paulo - SP – Brasil
3 Faculdade Santa Marcelina - São Paulo - SP – Brasil

Objective: Evaluate whether culture medium enriched with hyaluronic acid (EmbryoGlue®) improves implantation and clinical pregnancy rates (CPR) in frozen-thawed euploid embryo transfer cycles as compared to standard transfer medium in good prognosis patients.
Methods: This is a retrospective study conducted from March 2024 to May 2025 involving euploid frozen-thawed embryo transfer cycles, up to the third transfer at our facility. Patients with uterine abnormalities such as synechiae, fibroids, or adenomyosis, as well as endometrial thickness <7 mm, were excluded. Furthermore, patients over 42 years of age or with a body mass index (BMI) >30 were excluded. Another exclusion criterion was patients whose embryos were obtained through surgical sperm extraction. Endometrial preparation could be artificial or natural, and only euploid single embryo transfer cycles were included. Thus, 38 patients were included in the EmbryoGlue® (EG) group and 25 patients in the control group. A traditional incubator was used to cultivate the embryos, in a controlled environment (7.2% CO2, 5% O2, temperature 37°C). The embryo was placed in 1 ml of EG medium 1 hour before transfer. All transfers were performed after 3 hours of embryo thawing. We considered the implantation rate to be all Beta-hCG levels 9 days after transfer above 10IU/ml and CPR to be the percentage of pregnancies confirmed by ultrasound with the presence of a gestational sac
Results: At baseline, there were no statistically significant differences between the two study groups regarding age (p=0.2237) and BMI (p=0.7004). The EG group had 38 participants, with a mean age of 36.66 (3.87) years and a BMI of 23.46 (3.30) kg/m2. The control group had 25 participants, with a mean age of 37.75 (3.06) years and a BMI of 25.80 (3.33) kg/m2. An implantation rate of 52% was found in the EG group and 48% in the control group, with no statistically significant difference between the groups (p=0.9436). There was also no statistically significant difference between the groups regarding the clinical pregnancy rate, which was 34% in the EG group and 44% in the control group (p=0.1471). There was a predominance of artificial cycles in both groups with 76% in the EG group and 68% in the control group (p=0.2456)
Conclusion: The use of EG did not show improvement in CPR in patients with a good prognosis, despite a trend toward improved implantation rate (in our sample, with no statistical difference). The routine use of EG still requires further robust studies in different patient profiles to understand which populations would benefit and whether there will be a real improvement in the live birth rate.