JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):98
Poster Presentation
29^th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263685

P-86. Evaluation of sperm DNA Fragmentation after seminal processing by microfluidics (ZyMōt^®) and Discontinuous Density Gradient and Swim up

Larissa Jordão¹, Joana Melin¹, Ricardo Bertolla², Mariana Pereira Antoniassi¹

¹ UNIFESP - São Paulo - SP – Brasil
² Wayne State University – United States

Objective: To evaluate whether microfluidic techniques (ZyMōt^®) and discontinuous density gradient and swim up reduce sperm DNA fragmentation levels.
Methods: Samples from 37 patients recruited through clinical inclusion and exclusion criteria were tested. A comparative study of sperm DNA fragmentation was performed before and after the ZyMōt^® and discontinuous density gradient techniques associated with swim up. The seminal samples collected were evaluated in the Makler chamber, processed and used for the SCSA technique. Semen analysis (concentration, motility and morphology) was compared using paired t-test (values after ZyMōt^® x values after discontinuous density gradient + swim up). For DNA fragmentation data, the comparison was made using the paired Wilcoxon test (DFI values after ZyMōt^® x DFI values after discontinuous density gradient + swim up) and the paired t-test (HDS values after ZyMōt^® x HDS values after discontinuous density gradient + swim up). Results with p-values<0.05 were considered statistically significant.
Results: Significant differences were observed in sperm concentration, motility and morphology. It was found that the fresh semen presented an average sperm concentration of 94.14x10⁶ /mL, with 58.42x10⁶ /mL of progressively motile sperm and 1.71 of morphology. In turn, the seminal sample after processing by discontinuous density gradient + swim up, presented an average sperm concentration of 14.43x10⁶ /mL with 14.43 x 10⁶ /mL of progressively motile sperm and 2.29 of morphology. Regarding the sample after processing by the ZyMōt^® technique, an average sperm concentration of 28.14 x 10⁶ /mL was obtained, with 28.14x10⁶ /mL of progressively motile sperm and 2.86 of morphology. Furthermore, when comparing DNA fragmentation values, it was demonstrated that ZyMōt^® separated sperm with lower levels of DFI (mean 4.94; standard deviation 10.7) and HDS (mean 6.59; standard deviation 5.0).
Conclusion: The results obtained allowed us to consider that ZyMōt^® may be an effective technique due to the reduction in sperm DNA fragmentation levels. Furthermore, the data found could contribute new clinical and laboratory evidence for research on infertility, and can be applied in male healthcare.