JBRA Assisted Reproduction 2025;29(Suppl.2 SBRA 2025):216
Poster Presentation
29^th Annual Congress of the SBRA. São Paulo/SP - Brazil, 2025
doi: 10.5935/1518-0557.20263867

P-204. Semen analysis does not predict the pregnancy outcome in Assisted Reproduction

RoCamila Nogueira¹, Renato Fraietta¹, Ricardo Bertolla², Mariana Pereira Antoniassi¹

¹ UNIFESP - São Paulo – SP - Brasil
² Wayne State University -United States

Objective: To verify if seminal variables influence the results of assisted reproduction treatments (in vitro fertilization - IVF/ intracytoplasmic sperm injection - ICSI), the formation of embryos and blastocysts and pregnancy rates.
Methods: A total of 4,946 treatment cycles performed in a human reproduction clinic. between 2010 and 2020 were analyzed. After applying the inclusion and exclusion criteria, 2,037 cycles were selected. Data extracted from medical records included patient age, semen analysis results, and details of the assisted reproduction treatment. Student's t-test was used to compare groups in the univariate analysis. The multivariate statistics was performed by discriminant analysis to verify if the variables (semen, fertilization rate, blastocist formation and cleavage) predict the clinical outcome (pregnancy). All statistical analyses were conducted using SPSS software, version 18.0.
Results: When comparing female age between patients with and without pregnancy, a significant difference was observed (p<0.001). Similarly, the number of follicles retrieved, oocytes, and metaphase II (MII) oocytes also showed statistically significant differences between the groups (p=0.001, p<0.001, and p<0.001, respectively). A significant difference was also found in the MII and metaphase I (MI) rates (p=0.001 and p=0.003, respectively). In contrast, no significant association was observed between seminal variables and pregnancy outcomes. The number of injected oocytes and fertilized oocytes differed significantly between the groups (p<0.001for both). Embryo formation at D3 and D5 (p<0.001) and the number of embryos transferred at D5 (p<0.001) also showed significant differences. Finally, discriminant analysis revealed that the variables most strongly associated with pregnancy outcomes (beta-positive group) were female age, MII rate, number of oocytes retrieved, and fertilization rate, with the MII rate emerging as the strongest predictor.
Conclusion: Thus, the study suggests that seminal variables do not predict pregnancy outcomes following assisted reproduction treatments (IVF/ICSI).