JBRA Assist. Reprod. 2025;29(Suppl 1):41-41
POSTER PRESENTATION
doi: 10.5935/1518-0557.20250095
1Centro de Fertilidad y Reproducción Asistida, CEFRA S.A.C., Lima, Peru
Objective: In view of the available literature that describes that the pace of embryo development is linked to its genetic status, in this study, we aim to determine the euploidy of embryos that reach a biopsiable blastocyst stage at different timepoints.
Methods: This retrospective cohort study includes embryos of 909 ICSI cycles of infertile patients aged 25 to 47 years old. Additionally, 199 ICSI cycles, from our oocyte donation program, were analyzed separately. Enrolments took place between January 2019 and February 2025. Given the biopsy policy in our lab, good quality blastocysts are biopsied mostly around two timepoints: 120h and 144h with an average timing of 130h post ICSI. Therefore, two groups were compared: Blastocysts biopsied up to 130h post-ICSI and blastocysts biopsied after 130h post-ICSI. Moreover, blastocysts were grouped and analyzed by patient’s age. Additionally, good quality blastocysts, from our oocyte-donation program were evaluated. The euploidy of embryos was assessed by next-generation sequencing (NGS)-based preimplantation genetic testing for aneuploidies (PGT-A).
Results: Our analysis showed that blastocysts of patients < 40 years-old, biopsied ≤130h post-ICSI have a significantly higher euploidy rate than those biopsied >130h post-ICSI (43.7% vs 36.5%; p=0.0043).Similarly, blastocysts of patients ≥40 years-old, biopsied ≤130h post-ICSI have a significantly higher euploidy rate than those biopsied >130h post-ICSI (25.7% vs 15.7%; p=0.0006). Additionally, blastocysts from our oocyte donation program displayed the same characteristics. Thus, blastocysts biopsied ≤130h post-ICSI have a significantly higher euploidy rate than those biopsied >130h post-ICSI (61.1% vs 50.6%; p=0.0081).
Conclusion: In our setting, the data suggests that blastocysts that reach a developmental stage, suitable for mechanical biopsy, later than 130h post-ICSI are prone to a lower euploidy rate.