Table 2. Statistical comparison of semen parameters between the reference group (20-25 years) and other age groups (adjusted p-values).
Parameters 25-30 30-35 35-40 40-45 45-50 50-55 55-60 ≥60
Concentration 1 0.917 0.917 0.917 0.917 0.825 0.917 1
Progressive motility 0.086 0.014 0.013 0.002 <0.001 <0.001 <0.001 0.013
Total Sperm number 0.627 0.244 0.244 0.132 0.125 0.037 0.125 0.032
Morphology 1 1 1 1 1 1 1 1
Vitality 0.143 0.036 0.028 0.007 0.001 <0.001 <0.001 0.024
DFI 0.800 0.482 0.255 0.030 0.008 0.024 0.004 0.024

p-values were obtained from Mann-Whitney U tests comparing each age group to the reference group (20-25 years) and adjusted for multiple comparisons using the Holm correction. Bold values indicate statistical significance (adjusted p<0.05).