| Table I: Experiment 1: Time-dependent changes of motion parameters (CASA) and sperm viability (Hoechst staining) of human sperm incubated in HTF and 0.3% HSA under spontaneous conditions and following incubation with the calcium ionophore A23187 | ||||
| Hour | ||||
|---|---|---|---|---|
| 0a | 3 | 6 | 24 | |
| Concentration (×106/ml) | ||||
| Spontaneous | 20.0±0.0 | 19.2±0.8 | 18.8±1.3 | 16.4±3.6 |
| CaI |
19.0±1.7 | 17.2±1.9 | 17.8±3.3 | 16.0±2.5 |
| Motility (%) |
||||
| Spontaneous | 94.2±4.5 | 82.0±4.2 | 79.0±6.0 | 17.6±20.1 |
| CaI | 79.6±5.9 | 73.8±6.7 | 66.6±14.0 | 9.2±17.9 |
| Rapid cells (%) |
||||
| Spontaneous | 91.2±2.8 | 76.6±3.4 | 73.4±7.1 | 12.6±16.3 |
| CaI | 76.0±6.8 | 62.0±7.1 | 56.6±17.5 | 6.0±11.8 |
| VAP (μm/s) |
||||
| Spontaneous | 69.8±5.8 | 54.6±6.6 | 50.0±5.8 | 33.4±8.5 |
| CaI | 55.2±9.3 | 49.6±9.7 | 41.8±5.9 | 16.6±15.7 |
| VSL (μm/s) |
||||
| Spontaneous | 60.0±5.1 | 47.0±7.2 | 42.4±7.1 | 26.0±9.0 |
| CaI | 51.8±10.2 | 41.8±10.4 | 34.6±7.2 | 13.0±12.0 |
| VCL (μm/s) |
||||
| Spontaneous | 102.2±5.7 | 84.2±4.4 | 81.2±5.8 | 58.4±8.3 |
| CaI | 83.4±8.9 | 77.0±8.1 | 70.0±8.9 | 26.2±27.7 |
| Linearity (%) |
||||
| Spontaneous | 60.2±4.0 | 54.4±6.1 | 51.6±7.4 | 42.8±9.3 |
| CaI | 54.0±7.6 | 50.6±8.3 | 48.6±5.2 | 20.0±20.4 |
| Dead (%) |
||||
| Spontaneous | 5.0±2.7 | 5.0±2.1 | 8.0±3.5 | 14.0±6.1 |
| CaI | 5.0±2.7 | 7.4±3.2 | 10.8±5.8 | 12.6±6.5 |
a Time 0= following separation of the motile sperm fraction and resuspension.
b CaI= calcium ionophore A23187.
c For spontaneousand CaI-treated conditions all motion parameters had a significant decline over time p< 0.001
d For spontaneousand CaI-treated conditions viability had a significant decline over time (p< 0.01)