| Table 1. Oxidative stress markers (mean ±SD) in samples of infertile (n=20) and fertile men (n=10) treated with ascorbic acid (10.0 mM) or not. | ||||||
|
|
TBARS (nM MDA/108/h sperm) |
SOD (USod/g protein) |
CAT (UCat/mg protein) |
|||
|---|---|---|---|---|---|---|
| Infertile | Fertile | |||||
| Pre-freeze without ascorbic acid | 4.08 ± 0.05a |
2.56 ± 0.05a# | 8.10 ± 0.14ª |
11.86 ± 0.20a | 1.47 ± 0.07a |
4.78 ± 0.04a |
| Post-thaw without ascorbic acid | 7.96 ± 0.05b |
4.94 ± 0.0b | 3.17 ± 0.07b |
10.06 ± 0.10a | 3.41 ± 0.07b |
49.63 ± 2.79b |
| Post-thaw with ascorbic acid 10.0 mM |
5.92 ± 0.05c |
4.64 ± 0.07b | 6.52 ± 0.10c |
9.23 ± 0.14a | 4.50 ± 0.15b |
47.00 ± 1.42b |
MDA= malondialdehyde. #Values followed by different letters differ significantly, according to Wilcoxon test (p≤0.05) for each group. *Values statistically different from the fertile group, according to Mann-Whitney U test (p≤0.05).