Tabela III. Experiment 2: Annexin V binding to translocated membrane PS before and after cryopreservation and thawing in study and control groups.
Cells (%) 90% Percoll layer (high motility) 40% Percoll layer (low motility)
Prefreeze Postthaw P Prefreeze Postthaw P
Patients (study group)
Normal 79±4 52±5 0.002 67±4 43±4 0.005
Ann V +, live 12±3 26±2 0.13 20±3 28±2 0.01
Necrotic 8±2 22±3 0.02 12±3 28±2 0.007
Donors (control group)
Normal 87±2 70±2 0.01 69±2 39±2 0.01
Ann V +, live 10±1 23±1 0.01 20±2 37±2 0.01
Necrotic 3±1 7±1 0.05 11±1 24±1 0.05