| Tabela IV. Experiment 3: Annexin V binding assay results and motion parameters following prolonged incubation of fractionated motile spermatozoa. | |||||
| Incubation time
|
0 | 1 | 3 | 6 | 24 |
|---|---|---|---|---|---|
| Patients (study group) | |||||
| Annexin V assay | |||||
| Normal | 65±3a | 63±4 | 48±4 | 35±4a | 8±1 |
| Ann V+, live | 33±3b | 3 3 ±4 | 46±4. | 5 7±4b | 80±2 |
| Necrotic | 3±lc | 3±1 | 6±1 | 8±1 c | 12±2 |
| Motion parameters | |||||
| Motility | 83±2 d | 62± 12 | 29± 12 | 22±12d | 9±9 |
| Rapid sperm | 8 l±2e | 42± 12 | 23± 14 | 17±lle | 7±6 |
| Donors (control group) | |||||
| Annexin V assay | |||||
| Normal | 79±4f | 61 ±7 | 56±8 | 57±8 | 34±3i |
| Ann V+, live | 19±49g | 32±4 | 38±5 | 36±2g | 55±6 |
| Necrotic | 2±1 | 7±4 | 6±4 | 9±5 | 10±5 |
| Motion parameters | |||||
| Motility | 88±2h | 79+4 | 87±1 | 82±5 | 58±10 |
| Rapid spern | 85±1i | 75±5 | 82±2 | 78±5 | 44±9 |
Significant differences between groups with sarne superscript (P<0.05).