Neda Taghizabet, Soghra Bahmanpour, Nehleh Zarei Fard, Fatemeh Rezaei-Tazangi, Ashraf Hassanpour, Ebrahim Kharazi Nejad, Fereshteh Aliakbari, Farzaneh Dehghani
JBRA Assist. Reprod. 2022; 26 (3):508-521
Received March 28, 2021
Accepted September 30, 2021
Abstract
For the growth and survival of healthy follicles in the folliculogenesis process, several factors are needed, including endocrine and paracrine glands, and a regulated relationship of granulosa cells to oocytes. One of the most powerful methods of studying the folliculogenesis process is the culture of ovarian follicles and oogenesis into a completely controlled environment. Follicle culture systems are highly developed and rapidly evolving. However, the methods of separating the follicles, the cultivation techniques, the culture medium, and the dietary and hormonal supplements vary depending on the species studied. In this study, a literature review of follicular culture techniques was conducted and the heterogeneity among these key variables in follicular culture was investigated.
