Received May 31, 2010
Accepted August 14, 2010

Objective: The purpose of this study was to assess the effects of revitrification on the development of mice embryos using the Vitri-ingá® method. Methods: Randomized in vitro animal study was performed with Hybrid Balb C x C57 Bl/6 female mice. A total of 253 two-cell embryos were selected and randomly assigned to three groups: G1 fresh embryos; G2 vitrified embryos; and G3 revitrified embryos. After warming, vitrified and revitrified as well as fresh embryos were cultivated in Global® medium until its complete development. Blastocyst and hatching rates were analyzed and compared. Results: Blastocyst rates obtained in groups G1 (99.3%) and G2 (88.0%) were not statistically different, but were both significantly higher when compared to group G3. There were no significant differences in the hatching rates between the groups G2 and G3, but they were both significantly lower than group G1. Conclusion: Despite the cumulative damage caused by two successive vitrification processes, the results found suggest that the embryo revitrification protocol with the Vitri-ingá® method has the potential to be routinely used in assisted human reproduction laboratories.