Received March 11, 2011
Accepted March 28, 2011

Objective: Many embryos produced in vitro contain chromosomal abnormalities and have little potential for forming a viable pregnancy. The most commonly used method for preimplantation genetic diagnosis involves embryo biopsy on day 3 of development, followed by fluorescence in-situ hybridization analysis of 5-12 chromosomes. Here we report a new strategy for comprehensive chromosome screening in IVF embryos for poor reproductive prognosis patients, the Comparative Genome Hybridization by microarrays (aCGH). Methods: A total of 23 embryos, from 5 couples undergoing IFV treatment with history of unsuccessful attempts (>2 attempts) and miscarriages, were examined by aCGH after blastomere or trophectoderm biopsies. Results: The aCGH showed an efficiency of 95.6%. A total of 47.8% of embryos were euploid. Nine embryos were transferred to uterus without previous vitrification, reveling that 75% (6/8) of them produced fetal sac snd 50% of transferred embryos produced a fetus. Conclusion: The full chromosome complement screening method described overcomes the majority of the problems that limited earlier aneuploidy screening techniques and may finally allow preimplantation genetic screening to improve the implantation rates in poor prognosis patients.