Received April 28, 2011
Accepted June 12, 2011

Objective: In this study, the authors sought to test a simple and feasible technique, which measures the incidence of sperm with intact chromatin in a sample. Method: The tecnnique employs the type of dye kit Diff-Quik, routinely used in the analysis of sperm morphology, based on the intensity of pigmentation caused by the dye on the head of the sperm. We analyzed possible relationships between the incidence of sperm with fragmented chromatin and fertilization, cleavage and pregnancy. Results: Corroborating previous studies, significant relationships were observed in the rates of fertilization and cleavage, yielding a level of 30% as the limit of sperm with fragmented chromatin in the sample. Conclusion: This methodology was feasible to be used in routine laboratory, making it faster and cheaper the analogy of a predictive importance of seminal parameter and its plausible influence on the results of AHR.