Received May 26, 2010
Accepted October 02, 2010

Objective: Cryopreservation of embryos may be conducted by freezing slowly or quickly, among them the vitrification. This study aims to compare the development of embryos subjected to three different techniques of vitrification. Methods: Female mice F1 (C57bl/6xBlab) were subjected to a protocol of ovulation induction and placed with males. Eighty embryos were obtained by lavage of the uterine tube in the stage of eight cells to morula were divided randomly into four groups, twenty embryos Control group (A), twenty Cryotip ® group (B), twenty Cryotop ® group (C) and twenty Vitri-Inga® group (D). The Group A was grown in an incubator with 5% CO2 and 37 º C for 48 hours. The remaining embryos were vitrified according to the three techniques and thawed after 24 hours, being kept under the same conditions of the control group. Results: In group A, 18 embryos (90%) developed to blastocyst in group B, 15 embryos (75%), group C 14 embryos (70%) and group D 12 embryos (60%). The significance level was calculated based on the nonparametric test, AxB (p = 0.405), AXC (p = 0.236), AXD (p = 0.068), BXC (p = 1.000), BxD (p = 0.5) and CXD (p = 0.740) and the chi-square AxBxCxD (p = 0.184). Conclusion: There was no significant difference between control group and the other groups, nor between groups among themselves as to the embryonal blastocyst.