Received July 09, 2009
Accepted September 13, 2009

The morphology and sperm vitality are two of the characteristics evaluated in espermogramas that best correlated with fertility. This study aimed to compare the Bromophenol blue dye with the dye eosin for evaluation of vitality and Panótico of Blood for the evaluation of sperm morphology in humans. Twenty six samples of semen were collected by masturbation. For each sample two slides were prepared with 5ul of sperm in each. At first it added 5μl of eosin and the second 5μl of bromphenol blue. The prepared slides were left outdoors on a plate heated to 35° C for several minutes and then brought to the optical microscope. 200 sperm were counted for each slide and these were classified as to the vitality. For analysis of sperm morphology 10μl was fixed and stained by the technique of Panótico of Blood. To check whether there was statistical difference between treatments employed using the Student t test. The percentage of viable cells were similar, 82.4±81.68 to 83.76±83.06 and Eosin for Bromophenol blue, showed no statistical difference. When the Bromophenol blue dye was compared to Panótico of Blood to the morphological analysis, no statistical difference was seen between the normal sperm cells. Thus we can conclude that the Bromophenol blue dye is effective for assessment of human sperm morphology and vitality, can be used in laboratories in Human Reproduction.