Received November 04, 2008
Accepted December 23, 2008

Objective: The objective of this study was to propose the assessment of a new vitrification protocol through survival, fertilization and pregnancy rates after vitrification / thawing of oocytes. Material and methods: Among 54 patients included in this study, 258 oocytes were vitrified, using Vitri-Ingá protocol. The vitrification protocol was performed using two different vitrification solution concentrations and the thawing protocol was performed in three steps. Three hours after thawing the oocytes was submitted to ICSI and 72 hours later the embryos was transferred. Results: After thawing, 215 oocytes (83.3%) survived. ICSI was performed in 214 oocytes. After 72 hours, we have 164 (76.6%) embryos, 99 (63.5%) were top embryos. The pregnancy rate was 24.5%, resulting in 13 patients pregnant. Conclusion: The vitrification protocol used same to be a good option due to the high survival and fertilization rates after ICSI. Despite that, this protocol is an efficient alternative, fast and economical.