C Ruhlmann, A G Martínez, G Terrado, E D Rolla, R E Nicholson, A Cattáneo, P Mentasti, D Gnocchi
JBRA Assist. Reprod. 2003; 7 (1):23-25
Received August 09, 2022
Accepted March 31, 2023
Abstract
Embryo transfer in the blastocyst stage has been proposed as an alternative to increase the efficiency of ART. The aim of this study is to compare the results of day 3 embryo vs blastocyst stage transfer in patients with one or more previous unsuccessful attempts. Fifty-one blastocysts stage transfer cycles were prospectively tabulated and 89 day 3 transfer cycles were used as a concurrent and historical control group. Patients with no previus lVF or lCSl attempt, and over 39 years old or with less than 5 mature oocytes were excluded. Patients were stimulated under ovarian suppression with GnRH agonists, with recombinant FSH.
Ovulation was induced by a single dose of 10.000 lU of HCG 34-36h before oocyte retrieval. Five to eigth mature oocytes were inseminated. Zygotes were cultured in human tubal fluid medium. Blastocyst stage was achieved using co-culture with Vero cells. Statistically significant differences were found when comparing implantation rate (12.9% vs. 27.9% ) and delivery rate (21.3% vs. 43.1% ) between Day 3 and Blastocyst groups. Our results suggest that transfer of blastocyst stage embryos in patients with at least one previous unsuccessful attempt of lVF or lCSl may be beneficial.
