Received April 10, 2025
Accepted February 19, 2026

Objective: We compared post-cryopreserved outcomes of normozoospermic semen samples after cryopreservation by vitrification and liquid nitrogen vapor freezing, using the original and the simplified preservation media. Methods: Forty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into five aliquots: one served as non-cryopreserved control; two were vitrified using in-house (In-house-V) or sucrose media (Simp-V); and the last two aliquots were frozen in liquid nitrogen vapor, using commercial (Com-L) or sucrose media (Simp-L). Results: Sperm after cryopreservation regardless of the media and method used, significantly decreased in motility, viability, and increased ROS level without changes in sperm morphology and DNA fragmentation. Simplified sucrose freezing medium significantly increases post-thawed motility (57.6% (53.2-68.9) vs 34.5% (27.3-43.6) in Simp-L and Com-L) and viability (61.0% (52.0-67.8) vs 37.0% (29.3-46.0) in Simp-L and Com-L) in vapor freezing but significantly decrease post-thawed motility (58.8% (51.4-63.3) vs 77.8% (70.8-81.3) in Simp-V and In-house-V) and viability (59.0% (53.2-66.0) vs 75.5% (68.0-83.0) in Simp-V and In-house-V) in vitrification. A simplified medium does not affect sperm morphology, ROS level, and DNA fragmentation. Conclusions: In liquid nitrogen vapor freezing, a simplified medium significantly improved sperm motility and viability compared with commercial medium. In vitrification, the simplified medium gave inferior results on sperm motility and viability compared to the original preservation medium.