Received November 17, 2019
Accepted May 31, 2020

Objective: The aim of this study were investigated the possible influence of GDF9 polymorphisms on ovarian response in women undergoing controlled ovarian hyperstimulation for in vitro fertilization (IVF) with normal ovarian reserve. Methods: A cross-sectional study was performed with 67 women, with normal ovarian reserve and aged 30 to 39 years that were undergoing controlled ovarian hyperstimulation for IVF. We sequenced four polymorphisms of GDF9 (C398G, C447T, G546A and G646A) and analyzed their influence on follicular and oocytes results. Results: The mutant allele C398G decreased the total number of follicles > 17 mm (6.49 vs. 4.33, P=0.001), total number of follicles (10.11 vs. 7.33, P=0.032), number of MII oocytes retrieved, and the serum progesterone on the trigger day. The presence of C447T polymorphism was associated with a greater number of follicles between 12 and 14 mm on the day of r-hCG, and the polymorphism of G546A was associated with a lower level of serum progesterone on the trigger day. Conclusions: The GDF9 gene polymorphisms C398G and C447T negatively influence ovarian response in women undergoing controlled ovarian hyperstimulation. These finding show that in addition to playing a role in the early stages of folliculogenesis, the GDF9 polymorphisms have an important influence on the final stage of oocyte development.